SubtiBank SubtiBank
rlmCD [2019-01-30 12:55:28]
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rlmCD [2019-01-30 12:55:28]

rRNA methyltransferase
Locus
BSU06730
Isoelectric point
5.71
Molecular weight
51.65 kDa
Protein length
459 aa Sequence Blast
Gene length
1380 bp Sequence Blast
Function
23S rRNA maturation
Product
rRNA methyltransferase
Essential
no
E.C.
2.1.1.190
Synonyms
yerS,yefA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
737,603 → 738,982

The protein

Catalyzed reaction/ biological activity

  • methylation of U747 and U1939 in 23S rRNA PubMed
  • Protein family

  • COG2265
  • Paralogous protein(s)

    Cofactors

  • Fe-S cluster PubMed
  • Structure

  • 2BH2 (from E. coli, 32% identity) PubMed
  • Expression and Regulation

    Biological materials

    Mutant

  • MGNA-A931 (yefA::erm), available at the NBRP B. subtilis, Japan
  • BKE06730 (ΔrlmCD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
  • BKK06730 (ΔrlmCD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
  • References

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